Both the target DNA and the vector such as plasmid DNA can be cut by the same restriction enzyme, so that they produce complementary staggered ends, which are annealed together to get the rDNA (Figure 9.7).
PRODUCING RDNA BY STICKY-END LIGATION
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Both the target DNA and the vector such as plasmid DNA can be cut by the same restriction enzyme, so that they produce complementary staggered ends, which are annealed together to get the rDNA (Figure 9.7).