TOOLS OF RECOMBINANT DNA TECHNOLOGY
The basic tools of rDNA technology include various:
DNA ISOLATION AND SEQUENCING
The first step in the rDNA technology is the isolation of the desired gene of interest for which the DNA from the cell has been isolated. DNA Isolation DNA can be isolated by employing gentle methods of cell rupture. Cell walls if present are digested enzymatically (lysozyme treatment) and the cell membrane is solubilized using […]
INTRODUCTION
Recombinant DNA (rDNA) technology deals with the isolation and manipulation of DNA. DNA molecules from all organisms share the same chemical structure; they differ only in the sequence of nucleotides, consequently, a DNA from a foreign source can be linked to host DNA sequences, i.e., the DNAs of two different species can be linked to […]